Tile-level analysis. Region-of-interest flagging. Heat map overlay for the pathologist.
Whole-slide images can exceed 40,000 × 40,000 pixels at 40x magnification. Reviewing an entire WSI from top-left to bottom-right is not how pathologists work — they scan for regions of abnormality. Histolyx makes that scan explicit: tile-level classification across the full slide, ranked output, heat map overlay. The pathologist navigates to what matters instead of searching for it.
What Histolyx flags on whole-slide images.
Each flagged region is a candidate for pathologist review. Histolyx surfaces observations — the pathologist applies diagnostic judgment.
| Analysis type | What Histolyx marks | Output format |
|---|---|---|
| Tile classification | Each tile across the WSI is classified by cellular density and morphological regularity. Tiles with elevated atypical features are ranked and surfaced for review. | Heat map overlay on the WSI viewer. Top-N regions listed in structured report with coordinates and classification score. |
| Nuclear morphology flags | Regions with nuclear pleomorphism, increased nuclear-to-cytoplasmic ratio, or prominent nucleoli are flagged as candidates for closer review. | Regional bounding annotations on WSI. Score per region for pathologist triage. |
| Architectural irregularity | Loss of normal tissue architecture flagged — relevant for adenocarcinoma patterns, ductal carcinoma in situ features, and similar structural abnormalities. | Separate flag category in structured report. Tile coordinates included for direct navigation in WSI viewer. |
| Mitotic figure candidates | Candidate mitotic figures in high-power-equivalent tiles flagged for counting verification. Counts are estimates for pathologist review, not final grading inputs. | Per-tile count with region coordinates. Pathologist reviews and confirms before any grading determination. |
Histolyx digital pathology output is a pre-reading tool. All flagged regions are candidates for pathologist review. Diagnoses, grading determinations, and clinical conclusions are the pathologist's responsibility. Histolyx does not issue final diagnostic statements.
How Histolyx integrates with digital pathology lab workflow.
WSI file arrives from scanner
Whole-slide images are pushed from the slide scanner to Histolyx via your LIS or digital pathology platform connection. Supported formats include SVS (Aperio), TIFF, NDPI (Hamamatsu), and MRXS (MIRAX). No scanner replacement required.
Tile-level analysis runs
Histolyx tiles the WSI and classifies each tile. Regions of elevated cellular density, nuclear irregularity, and architectural change are ranked. The analysis runs without requiring high-magnification zoom from the pathologist — the model operates at a level equivalent to 20x–40x objective examination.
Heat map and report generated
A heat map overlay is returned to the WSI viewer, highlighting the ranked regions. A structured report lists the top flagged coordinates with classification descriptions. The pathologist receives both outputs when they open the slide.
Pathologist navigates and diagnoses
Instead of reviewing the entire slide from the beginning, the pathologist navigates directly to flagged regions using the heat map. They examine each area, apply their expertise, and issue the final pathology report. The pre-read directs attention; the diagnosis belongs to the pathologist.